The Methods and Reagents Wiki

 

Antibody stripping buffer

Page history last edited by Tom Anderson 1 yr ago

For stripping antibodies from western blots, use:

 

  • 10 mM NaOH

 

For a stronger version, use:

 

  • 100 mM NaOH

How long should you use it for? Rocking or just sitting there? At what temperature? How do you wash it afterwards, before re-probing?

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